BISCUIT Subcommands
Usage for all BISCUIT subcommands as of BISCUIT version 1.4.0 (printed with either biscuit
or biscuit help
).
Program: BISCUIT (BISulfite-seq CUI Toolkit)
Version: 1.5.0
Contact: Jacob Morrison <jacob.morrison@vai.org>
Usage: biscuit <command> [options]
Command:
-- Read mapping
index Index reference genome sequences in the FASTA format
align Align bisulfite treated short reads using adapted BWA-mem
algorithm
-- BAM operation
tview Text alignment viewer with bisulfite coloring
bsstrand Validate/correct bisulfite conversion strand label (YD tag)
bsconv Summarize/filter reads by bisulfite conversion (ZN tag)
cinread Print cytosine-read pair in a long form
-- Base summary
pileup Pileup cytosine and mutations
vcf2bed Convert VCF to BED file
mergecg Merge C and G in CpG context
-- Epireads
epiread Convert BAM to epibed format
rectangle Convert epiread format to rectangle format
asm Test allele-specific methylation
-- Other
version Print BISCUIT and library versions
help Print usage and exit
qc Generate QC files from BAM
bc Extract barcodes from FASTQ files